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Carbohydrate Chains and Their Binding Sites in Mistletoe Lectin I

Stoeva, S. and Maier, T. and Soler, M. and Voelter, W.. (1999) Carbohydrate Chains and Their Binding Sites in Mistletoe Lectin I. Polish Journal of Chemistry, 73 (1). pp. 125-133.

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Official URL: http://edoc.unibas.ch/51406/

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Abstract

The carbohydrate moieties, linked to mistletoe lectin 1 (ML-1) are characterized by enzymatic digestion of the individual A and B chains (MLA and MLB), HPLC separation of the (glyco)peptide digests and their sequence and mass spectrometric analysis. All carbohydrate chains are linked via Asn residues to the MLA protein molecule. A xylomannose-type oligosaccharide (1) structure (Man3Xyl1Fuc1GlcNac2) is attached to the Asn112 residue of the A chain. Three different glycosylation sites are identified for the B chain: oligosaccharide II (Man6GlcNac2) is found to be linked to Asn96 as well as Asn136 residues. In homologous mistletoe lectins, isolated from different harvest batches, oligosaccharide III (Man5GlcNac2) is attached to Asn136 instead of oligosaccharide II**.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Maier)
UniBasel Contributors:Maier, Timm
Item Type:Article
Article Subtype:Research Article
ISSN:0137-5083
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:26 Oct 2017 12:45
Deposited On:26 Oct 2017 12:45

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