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The human peripheral subunit-binding domain folds rapidly while overcoming repulsive Coulomb forces

Arbely, Eyal and Neuweiler, Hannes and Sharpe, Timothy D. and Johnson, Christopher M. and Fersht, Alan R.. (2010) The human peripheral subunit-binding domain folds rapidly while overcoming repulsive Coulomb forces. Protein Science, 19 (9). pp. 1704-1713.

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Official URL: http://edoc.unibas.ch/47490/

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Abstract

Peripheral subunit binding domains (PSBDs) are integral parts of large multienzyme complexes involved in carbohydrate metabolism. PSBDs facilitate shuttling of prosthetic groups between different catalytic subunits. Their protein surface is characterized by a high density of positive charges required for binding to subunits within the complex. Here, we investigated folding thermodynamics and kinetics of the human PSBD (HSBD) using circular dichroism and tryptophan fluorescence experiments. HSBD was only marginally stable under physiological solvent conditions but folded within microseconds via a barrier-limited apparent two-state transition, analogous to its bacterial homologues. The high positive surface-charge density of HSBD leads to repulsive Coulomb forces that modulate protein stability and folding kinetics, and appear to even induce native-state movement. The electrostatic strain was alleviated at high solution-ionic-strength by Debye-H`uckel screening. Differences in ionic-strength dependent characteristics among PSBD homologues could be explained by differences in their surface charge distributions. The findings highlight the trade-off between protein function and stability during protein evolution.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Biophysics Facility (Sharpe)
UniBasel Contributors:Sharpe, Timothy
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cambridge University Press
ISSN:0961-8368
e-ISSN:1469-896X
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:30 Nov 2017 07:27
Deposited On:30 Nov 2017 07:26

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