Ölmezer, Gizem. Understanding the in vivo functions of Holliday junction resolvase Yen1. 2016, PhD Thesis, University of Basel, Faculty of Science.
Restricted to Repository staff only until 10 November 2018.
Official URL: http://edoc.unibas.ch/diss/DissB_11958
In this work, we used budding yeast as a model to explore whether additional, Mus81-independent functions of Yen1 exist, potentially outside the context of canonical Holliday junction resolution. To this end, we investigated the reported genetic interactome of YEN1. We disproved a purported genetic interaction between YEN1 and PBY1, linking wrongly assigned PBY1 interactions to MUS81-MMS4 instead. More significantly, addressing a synthetic sick interaction between YEN1 and the conserved nuclease-helicase DNA2, we identify a first unique function of Yen1 in targeting replication, as opposed to recombination, intermediates. Furthermore, we uncover a novel role for the elusive Dna2 helicase activity in the recovery of stalled replication intermediates. Our findings provide new insight into the replication stress response in eukaryotes.
|Advisors:||Gasser, Susan and Rass, Ulrich and Schär, Primo|
|Faculties and Departments:||09 Associated Institutions > Friedrich Miescher Institut FMI > Functional organization of the nucleus (Gasser)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||1 Online-Ressource (131 Seiten)|
|Last Modified:||21 Dec 2016 09:39|
|Deposited On:||21 Dec 2016 09:38|
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