Mansouri, Maysam. Multigene delivery to mammalian cells and its applications in cell biology. 2016, PhD Thesis, University of Basel, Faculty of Science.
Restricted to Repository staff only until 1 August 2017.
Official URL: http://edoc.unibas.ch/diss/DissB_11781
The thesis is organized in five chapters. The first chapter provides an introduction to available and common strategies for multigene expression in mammalian cells as well as some of the most important applications of multigene delivery in biological research. The chapters two, three, and four present original research reports covering our established system, an optimized protocol necessary for production and utilization of the system and development of some software for analysis of acquired images by our system. In chapter two, Mansouri et al. (2016) present the main project including establishment of MultiPrime and its diverse applications in cell biology. In chapter three, Mansouri et al. (manuscript in preparation) describe an optimized protocol to generate MultiPrime baculovirus particles that harbor complete gene constructs. In chapter four, Rizk et al. (2014 and 2015) present Squassh, Squassh3C and SquasshAnalyst, three user-friendly software modules that enable segmentation and quantification of subcellular structures of fluorescence microscopy images. In the last Chapter, I discuss and summarize our results and refer to the limitations of our system and I propose possible future directions for genetic manipulation of cells.
|Advisors:||Ballmer-Hofer, Kurt and Spiess, Martin|
|Faculties and Departments:||05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spiess)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||1 Online-Ressource (110 Seiten)|
|Last Modified:||23 Sep 2016 08:54|
|Deposited On:||23 Sep 2016 08:49|
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