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Molecular determinants of nucleosome retention at CpG-rich sequences in mouse spermatozoa

Erkek, Serap and Hisano, Mizue and Liang, Ching-Yeu and Gill, Mark and Murr, Rabih and Dieker, Jürgen and Schübeler, Dirk and van der Vlag, Johan and Stadler, Michael B. and Peters, Antoine H. F. M.. (2013) Molecular determinants of nucleosome retention at CpG-rich sequences in mouse spermatozoa. Nature structural & molecular biology, 20 (7). pp. 868-875.

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Official URL: http://edoc.unibas.ch/40111/

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Abstract

In mammalian spermatozoa, most but not all of the genome is densely packaged by protamines. Here we reveal the molecular logic underlying the retention of nucleosomes in mouse spermatozoa, which contain only 1% residual histones. We observe high enrichment throughout the genome of nucleosomes at CpG-rich sequences that lack DNA methylation. Residual nucleosomes are largely composed of the histone H3.3 variant and are trimethylated at Lys4 of histone H3 (H3K4me3). Canonical H3.1 and H3.2 histones are also enriched at CpG-rich promoters marked by Polycomb-mediated H3K27me3, a modification predictive of gene repression in preimplantation embryos. Histone variant–specific nucleosome retention in sperm is strongly associated with nucleosome turnover in round spermatids. Our data show evolutionary conservation of the basic principles of nucleosome retention in mouse and human sperm, supporting a model of epigenetic inheritance by nucleosomes between generations.
Faculties and Departments:09 Associated Institutions > Friedrich Miescher Institut FMI > Epigenetics > Epigenetic control of mouse germ cell and early embryonic development (Peters)
UniBasel Contributors:Peters, Antoine
Item Type:Article, refereed
Publisher:Nature Publ. Group
ISSN:1545-9993
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:30 Jun 2016 11:00
Deposited On:29 Jan 2016 07:44

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