edoc

Carrier Mediated Distribution System (CAMDIS): a new approach for the measurement of octanol/water distribution coefficients

Wagner, Bjoern and Fischer, Holger and Kansy, Manfred and Seelig, Anna and Assmus, Frauke. (2015) Carrier Mediated Distribution System (CAMDIS): a new approach for the measurement of octanol/water distribution coefficients. European journal of pharmaceutical sciences, 68. pp. 68-77.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/40008/

Downloads: Statistics Overview

Abstract

Here we present a miniaturized assay, referred to as Carrier-Mediated Distribution System (CAMDIS) for fast and reliable measurement of octanol/water distribution coefficients, log D(oct). By introducing a filter support for octanol, phase separation from water is facilitated and the tendency of emulsion formation (emulsification) at the interface is reduced. A guideline for the best practice of CAMDIS is given, describing a strategy to manage drug adsorption at the filter-supported octanol/buffer interface. We validated the assay on a set of 52 structurally diverse drugs with known shake flask log D(oct) values. Excellent agreement with literature data (r(2) = 0.996, standard error of estimate, SEE = 0.111), high reproducibility (standard deviation, SD < 0.1 log D(oct) units), minimal sample consumption (10 μL of 100 μM DMSO stock solution) and a broad analytical range (log D(oct) range = -0.5 to 4.2) make CAMDIS a valuable tool for the high-throughput assessment of log D(oc)t.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Biophysical Chemistry (Seelig A)
UniBasel Contributors:Seelig-Löffler, Anna
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:0928-0987
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:30 Jun 2016 11:00
Deposited On:30 May 2016 09:13

Repository Staff Only: item control page