Nestorov, Peter Dobrinov. The role of polycomb repressive complex 2 in mouse preimplantation development. 2015, PhD Thesis, University of Basel, Faculty of Science.
Restricted to Repository staff only until 1 May 2017.
Available under License CC BY (Attribution).
Official URL: http://edoc.unibas.ch/diss/DissB_11248
I demonstrated that genetic ablation of core members of PRC2 has an effect on H3K27me3 in vivo, and leads to a developmental and transcriptional response in late oocytes and early embryos. Furthermore, the observed mutant phenotypes revealed a dosage-dependent requirement for PRC2/H3K27me3 in the preimplantation embryo. I also found genetic evidence for an interplay between the two major Polycomb complexes, PRC1 and PRC2, in preimplantation embryos. I further described the transcriptional dynamics during early embryonic development of genes encoding chromatin modifiers. This single-cell profiling study highlighted the existence of maternal and embryonic variants of the major chromatin modifying complexes.
In summary, my work reveals an important role of chromatin-based regulation in the preparation and acquisition of totipotency in vivo, manifested by a dosage-dependent PRC2/H3K27me3 requirement during the maternal-to-zygotic transition.
|Committee Members:||Zeller, Rolf|
|Faculties and Departments:||09 Associated Institutions > Friedrich Miescher Institut FMI > Epigenetic control of mouse germ cell and early embryonic development (Peters)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||184 p.|
|Last Modified:||30 Jun 2016 10:57|
|Deposited On:||15 Jun 2015 08:23|
Repository Staff Only: item control page