Toughraï, Smahan. Functional surfaces through biomimetic block copolymer membranes. 2015, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_11147
The versatility of polymer chemistry allows the fine-tuning of biomimetic membranes in solution and on solid supports. Methacrylate-based amphiphilic triblock copolymers poly (2-hydroxyethyl methacrylate)-b-poly (butyl methacrylate)-b-poly (2-hydroxyethyl methacrylate) PHEMA-b-PBMA-b-PHEMA were designed in solution and on gold surfaces. By varying the hydrophilic to hydrophobic ratio as well as the chain length, the polymers self-assembled into nanoparticles and micelles in solution. The micelles were used to encapsulate and release hydrophobic model payloads, showing their potential use as intracellular drug delivery systems. Also, artificial planar membranes as mimics of natural membranes were synthesized directly from gold surfaces. Upon the variations in thickness and packing density, potential incorporation of membrane proteins was shown at a determined grafting density. Upon insertion of those proteins, this system may find its application as biosensing devices.
In solution, the nanostructures were characterized by using a wide range of methodologies including static and dynamic light scattering, transmission electron microscopy, ThioGlo detection, UV-vis spectroscopy, fluorescence spectroscopy and fluorescence correlation microscopy. On solid supports, atomic force microscopy and surface plasmon resonance along with neutron reflectivity were used to gain insights into morphology, homogeneity, grafting density and thickness of the layers. To demonstrate the planar membranes’ biomimetic potential, they were incubated with different channel proteins: Outer Membrane Protein F, Aquaporin Z and alpha-hemolysin. Occurring interactions were detected by in-situ ATR-FTIR and electrochemical impedance spectroscopy. In summary, this thesis might impact fundamental membrane science as well as prospective biotechnological applications.
|Advisors:||Meier, Wolfgang Peter|
|Committee Members:||Bruns, Nico and Textor, Marcus|
|Faculties and Departments:||05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||142 p.|
|Last Modified:||30 Jun 2016 10:57|
|Deposited On:||20 Feb 2015 09:53|
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