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Global 3' UTR shortening has a limited effect on protein abundance in proliferating T cells

Gruber, Andreas R. and Martin, Georges and Müller, Philipp and Schmidt, Alexander and Gruber, Andreas J. and Gumienny, Rafal and Mittal, Nitish and Jayachandran, Rajesh and Pieters, Jean and Keller, Walter. (2014) Global 3' UTR shortening has a limited effect on protein abundance in proliferating T cells. Nature communications, 5 (5465).

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Official URL: http://edoc.unibas.ch/dok/A6328956

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Abstract

Alternative polyadenylation is a cellular mechanism that generates mRNA isoforms differing in their 3' untranslated regions (3' UTRs). Changes in polyadenylation site usage have been described upon induction of proliferation in resting cells, but the underlying mechanism and functional significance of this phenomenon remain largely unknown. To understand the functional consequences of shortened 3' UTR isoforms in a physiological setting, we used 3' end sequencing and quantitative mass spectrometry to determine polyadenylation site usage, mRNA and protein levels in murine and human naive and activated T cells. Although 3' UTR shortening in proliferating cells is conserved between human and mouse, orthologous genes do not exhibit similar expression of alternative 3' UTR isoforms. We generally find that 3' UTR shortening is not accompanied by a corresponding change in mRNA and protein levels. This suggests that although 3' UTR shortening may lead to changes in the RNA-binding protein interactome, it has limited effects on protein output.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Bioinformatics (Zavolan)
05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Bioinformatics (van Nimwegen)
05 Faculty of Science > Departement Biozentrum > Infection Biology > Biochemistry (Pieters)
UniBasel Contributors:Zavolan, Mihaela and Gruber, Andreas R. and Martin, Georges and Gruber, Andreas J. and Gumienny, Rafal Wojciech and Pieters, Jean and Mittal, Nitish and Keller, Walter and Jayachandran, Rajesh and Schmidt, Alexander and van Nimwegen, Erik
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Nature Publishing Group
ISSN:2041-1723
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:28 Apr 2017 13:32
Deposited On:06 Feb 2015 09:59

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