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Promoters, transcripts, and regulatory proteins of Mungbean yellow mosaic geminivirus

Shivaprasad, P. V. and Akbergenov, Rashid and Trinks, Daniela and Rajeswaran, R. and Veluthambi, K. and Hohn, Thomas and Pooggin, Mikhail M.. (2005) Promoters, transcripts, and regulatory proteins of Mungbean yellow mosaic geminivirus. Journal of virology, Vol. 79, H. 13. S. 8149-8163.

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Official URL: http://edoc.unibas.ch/dok/A6205231

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Abstract

Geminiviruses package circular single-stranded DNA and replicate in the nucleus via a double-stranded intermediate. This intermediate also serves as a template for bidirectional transcription by polymerase II. Here, we map promoters and transcripts and characterize regulatory proteins of Mungbean yellow mosaic virus-Vigna (MYMV), a bipartite geminivirus in the genus Begomovirus. The following new features, which might also apply to other begomoviruses, were revealed in MYMV. The leftward and rightward promoters on DNA-B share the transcription activator AC2-responsive region, which does not overlap the common region that is nearly identical in the two DNA components. The transcription unit for BC1 (movement protein) includes a conserved, leader-based intron. Besides negative-feedback regulation of its own leftward promoter on DNA-A, the replication protein AC1, in cooperation with AC2, synergistically transactivates the rightward promoter, which drives a dicistronic transcription unit for the coat protein AV1. AC2 and the replication enhancer AC3 are expressed from one dicistronic transcript driven by a strong promoter mapped within the upstream AC1 gene. Early and constitutive expression of AC2 is consistent with its essential dual function as an activator of viral transcription and a suppressor of silencing.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Ehemalige Einheiten Umweltwissenschaften > Molekulare Pflanzenvirologie (Hohn)
UniBasel Contributors:Pooggin, Mikhail
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Springer
ISSN:0022-538X
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:31 Jan 2014 09:49
Deposited On:31 Jan 2014 09:49

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