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Mechanism of ribosome shunting in Rice tungro bacilliform pararetrovirus

Pooggin, Mikhail M. and Ryabova, Lyubov A. and He, Xiaoyuan and Fütterer, Johannes and Hohn, Thomas. (2006) Mechanism of ribosome shunting in Rice tungro bacilliform pararetrovirus. RNA, Vol. 12, H. 5. S. 841-850.

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Official URL: http://edoc.unibas.ch/dok/A6205228

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Abstract

In plant pararetroviruses, pregenomic RNA serves both as a template for replication through reverse transcription and a polysictronic mRNA. This RNA has a complex leader sequence preceding the first large ORF. The leader contains multiple short ORFs and strong secondary structure, both inhibiting ribosome scanning. Translation on this RNA is initiated by shunting, in which scanning ribosomes bypass a large portion of the leader with the inhibitory secondary structure and short ORFs. In Cauliflower mosaic virus (CaMV), the ribosome shunting mechanism involves translation of the 5'-proximal short ORF terminating in front of the secondary structure that appears to force ribosomes to take off and resume scanning at a landing site downstream of the structure. Using two plant protoplast systems and shunt-competent wheat-germ extracts, we demonstrate that in Rice tungro bacilliform virus (RTBV) shunting also depends on the first short ORF followed by strong secondary structure. Swapping of the conserved shunt elements between CaMV and RTBV revealed the importance of nucleotide composition of the landing sequence for efficient shunting. The results suggest that the mechanism of ribosome shunting is evolutionary conserved in plant pararetroviruses.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Ehemalige Einheiten Umweltwissenschaften > Molekulare Pflanzenvirologie (Hohn)
UniBasel Contributors:Pooggin, Mikhail
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Cambridge University Press
ISSN:1355-8382
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:31 Jan 2014 09:49
Deposited On:31 Jan 2014 09:49

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