Pereira Nogueira, Elisa Sofia. Novel approaches for the creation of artificial metalloenzymes. 2013, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_10645
The key focuses of this research were the development of (i) novel purification strategies to simplify the challenge of producing functional protein (streptavidin), in sufficient quantity and appropriate purity for high-throughput screening of organometallic moieties; (ii) a reiterative approach to protein expression: re-design of streptavidin construct, moving to a more complex expression system, Pichia pastoris, to increase the production of streptavidin and simplify the purification process; and (iii) a novel biomolecular scaffold, human carbonic anhydrase II, for the creation of a new artificial metalloenzyme.
This thesis addresses the issues and feasibility of purification and expression processes and, highlights bottlenecks that arose using these technologies, key learning's and where to go from now.
|Advisors:||Ward, Thomas R.|
|Committee Members:||Grogan, Gideon J.|
|Faculties and Departments:||05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||187 S.|
|Last Modified:||30 Jun 2016 10:54|
|Deposited On:||20 Jan 2014 15:57|
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