Biebricher, Andreas and Hirano, Seiki and Enzlin, Jacqueline H. and Wiechens, Nicola and Streicher, Werner W. and Huttner, Diana and Wang, Lily H.-C. and Nigg, Erich A. and Owen-Hughes, Tom and Liu, Ying and Peterman, Erwin and Wuite, Gijs J. L. and Hickson, Ian D.. (2013) PICH: a DNA Translocase Specially Adapted for Processing Anaphase Bridge DNA. Molecular cell, Vol. 51, H. 5. pp. 691-701.
Full text not available from this repository.
Official URL: http://edoc.unibas.ch/dok/A6174405
Downloads: Statistics Overview
Abstract
The Plk1-interacting checkpoint helicase (PICH) protein localizes to ultrafine anaphase bridges (UFBs) in mitosis alongside a complex of DNA repair proteins, including the Bloom's syndrome protein (BLM). However, very little is known about the function of PICH or how it is recruited to UFBs. Using a combination of microfluidics, fluorescence microscopy, and optical tweezers, we have defined the properties of PICH in an in vitro model of an anaphase bridge. We show that PICH binds with a remarkably high affinity to duplex DNA, resulting in ATP-dependent protein translocation and extension of the DNA. Most strikingly, the affinity of PICH for binding DNA increases with tension-induced DNA stretching, which mimics the effect of the mitotic spindle on a UFB. PICH binding also appears to diminish force-induced DNA melting. We propose a model in which PICH recognizes and stabilizes DNA under tension during anaphase, thereby facilitating the resolution of entangled sister chromatids.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg) |
---|---|
UniBasel Contributors: | Nigg, Erich A. |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Cell Press |
ISSN: | 1097-2765 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Related URLs: | |
Identification Number: |
|
Last Modified: | 25 Oct 2013 08:33 |
Deposited On: | 25 Oct 2013 08:33 |
Repository Staff Only: item control page