Burazerovic, Sabina. Streptavidin-based nanostructures : from bulk to surface-confined assembly studies. 2013, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_10484
This thesis was dedicated to program the self-organization of mature wild-type streptavidin by using linear tetrabiotinylated and trifurcated hexabiotinylated connectors.
Self-organization studies in bulk solution demonstrated that streptavidin combined with a linear tetrabiotinylated connector spontaneously assembled into a one-dimensional streptavidin-based block copolymer. In the presence of calcium ions, the fibrils formed bundles that served as a template for the nucleation, the growth and the assembling of calcite microcrystals. This hierarchical self-assembly process yielded millimeter-sized one-dimensional mineralized protein matrices. Both mineralized and naked one-dimensional streptavidin-based block co- polymers were imaged by electron microscopy.
With the aim of obtaining well-defined and monodisperse nanostructures, a step-by- step assembly approach was investigated. Designed mixed bis-biotinylated monolayers on gold were used to anchor streptavidin, which was successively exposed to linear tetrabiotinylated or trifurcated hexabiotinylated connectors and streptavidin. The stepwise elongation process was followed in situ by surface plasmon resonance and quartz crystal microbalance with dissipation monitoring. The size-controlled immobilized streptavidin-based fibrils were scrutinized by atomic force microscopy in the hydrated state.
|Advisors:||Ward, Thomas R.|
|Committee Members:||Meier, Wolfgang Peter|
|Faculties and Departments:||05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||166 S.|
|Last Modified:||30 Jun 2016 10:53|
|Deposited On:||03 Sep 2013 10:23|
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