Urwyler, Prabitha. Polymeric micro-cantilever sensors for biomedical applications. 2013, PhD Thesis, University of Basel, Faculty of Medicine.
Official URL: http://edoc.unibas.ch/diss/DissB_10415
In a second step, the injection molded micro-cantilevers were characterized for their mechanical and morphological properties. Their performance was similar to the established silicon cantilevers with Q-factors in the range of 10-20. Nanoindentation techniques were used to evaluate the elastic modulus of the micro-cantilevers. Synchrotron radiation-based scanning small- and wide-angle X-ray scattering (SAXS, WAXS) techniques were used to quantify crystallinity and anisotropy in polymer micro-cantilevers with micrometer resolution in real space. SAXS measurements confirmed the lamellar nature of the injection-molded semi-crystalline micro-cantilevers showing the expected strong degree of anisotropy along the injection direction. The homogenous cantilever material exhibits a lamellar periodicity increasing with mold temperature but not with injection speed.
In a last step, we demonstrate that polypropylene cantilevers can be used as biosensors for medical purposes in the same manner as the established silicon ones to detect single-stranded DNA sequences and metal ions in real-time. A differential signal of 7 nm was detected for the hybridization of 1 µM complementary DNA sequences. For 100 nM copper ions the differential signal was found to be (36 ± 5) nm. Nano-mechanical sensing of medically relevant, nanometer-size species is essential for fast and efficient diagnosis.
The developed low-cost micro-cantilever arrays adapted to the geometric requirements of the Cantisens platform will significantly widen the spectrum of applications. Rather simple further adaptations to the fabrication process will allow an easy tailoring for their application in other systems. It may result in dedicated bedside systems for the benefit of patients.
|Committee Members:||Gobrecht, Jens|
|Faculties and Departments:||03 Faculty of Medicine > Departement Biomedical Engineering > Biomaterials Science Center > Materialwissenschaft (Müller)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||55 Bl.|
|Last Modified:||30 Jun 2016 10:53|
|Deposited On:||12 Jul 2013 12:40|
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