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Chromatin measurements reveal contributions of synthesis and decay to steady-state mRNA levels

Tippmann, Sylvia C. and Ivanek, Robert and Gaidatzis, Dimos and Schöler, Anne and Hoerner, Leslie and van Nimwegen, Erik and Stadler, Peter F. and Stadler, Michael B. and Schübeler, Dirk. (2012) Chromatin measurements reveal contributions of synthesis and decay to steady-state mRNA levels. Molecular systems biology, Vol. 8. S. 593.

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Official URL: http://edoc.unibas.ch/dok/A6043861

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Abstract

Messenger RNA levels in eukaryotes are controlled by multiple consecutive regulatory processes, which can be classified into two layers: primary transcriptional regulation at the chromosomal level and secondary, co- and post-transcriptional regulation of the mRNA. To identify the individual contribution of these layers to steady-state RNA levels requires separate quantification. Using mouse as a model organism, we show that chromatin features are sufficient to model RNA levels but with different sensitivities in dividing versus postmitotic cells. In both cases, chromatin-derived transcription rates explain over 80% of the observed variance in measured RNA levels. Further inclusion of measurements of mRNA half-life and microRNA expression data enabled the identification of a low quantitative contribution of RNA decay by either microRNA or general differential turnover to final mRNA levels. Together, this establishes a chromatin-based quantitative model for the contribution of transcriptional and post-transcriptional processes to steady-state levels of messenger RNA.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Bioinformatics (van Nimwegen)
UniBasel Contributors:van Nimwegen, Erik
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:EMBO
ISSN:1744-4292
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:16 Aug 2013 07:29
Deposited On:01 Feb 2013 08:39

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