Nobs, Lionel. The impact of Cyclin D1 on proliferating cells in the intact and injured mouse cortex. 2012, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_10208
In this study, I took advantage of mice genetically deficient in the Cyclin D1 gene to study its function in proliferating cells of the intact and injured cortex. I could show that the postnatal proliferation of microglia is completely impaired by the absence of Cyclin D1. In contrast, the proliferation of oligodendrocyte progenitor cells (OPCs) was independent of Cyclin D1 at early postnatal stages and only reduced during adult stages. This finding suggests a switch in the requirement for distinct cell cycle proteins driving proliferation of OPCs during development and in the adult. Using an injury model leading to local neurodegeneration, I could show that the deficiency for Cyclin D1 reduces the size of the lesion three days following insult to the adult brain cortex. In parallel, the injury-induced Proliferation was significantly reduced within the lesion site. A closer examination of the glial cell types within the neurodegenerative area revealed that the proliferation of microglia and OPCs was impaired in the Cyclin D1 knockout animals. Finally, I provided evidence that Cdk4 but not Cdk2 or Cdk6 are required for injury-induced proliferation of OPCs thus indicating Cdk4 most likely to be the interaction partner of Cyclin D1.
|Committee Members:||Nitsch, Cordula|
|Faculties and Departments:||03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Cellular Neurobiology (Atanasoski)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||104 S.|
|Last Modified:||30 Jun 2016 10:51|
|Deposited On:||18 Jan 2013 10:13|
Repository Staff Only: item control page