Noij, Peter. Label-free detection of noncoding RNAs. 2012, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_10046
We have demonstrated the detection of the biomarkers coagulation factor VII messengerRNA transcripts (F7) and microRNA-122 (miR-122) in proof of concept experiments with spike-in target in totalRNA background and cell lysates as well as the detection out of biologically relevant cell lysate samples with naturally occurring targets. Furthermore we have demonstrated the detection of miR-122 in acetaminophen treated rats’ plasma and therefore prove the cantilever array technology to be a useful tool for the label-free direct detection of noncoding RNAs.
Biacore’s surface plasmon resonance (SPR) system, the gold standard for label-free quantitative biomolecular interaction analysis, was used as reference parallel to the cantilever array technology (CLA). We compared the two fundamentally different systems, where the SPR measures the change in index of refraction in a boundary layer on the sensor surface and the CLA measures surface stress, in order to further support the interpretation of our results. The SPR can distinguish specific binding mostly by kinetic analysis and less by amplitude changes whereas the CLA offers to date less kinetic information but direct observation of amplitude changes. Plus, the CLA’s dynamic range and sensitivity is higher.
|Committee Members:||Hegner, Martin|
|Faculties and Departments:||05 Faculty of Science > Departement Physik > Physik > Nanomechanik (Meyer)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||76 S.|
|Last Modified:||30 Jun 2016 10:51|
|Deposited On:||18 Jan 2013 09:38|
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