Martina, Enrico. The last tenascin : insights on tenascin-W functions and regulation. 2012, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_10081
We also report that tenascin-W is upregulated during osteogenic differentiation of human bone marrow derived mesenchymal stem cells, fitting with its expression during bone development reported in chicken and mice.
In order to shed light on the mechanism of regulation, we studied the human tenascin-W promoter. We found a putative GATA binding site in this promoter that enhances reporter gene activity when deleted or mutated. We used a labeled DNA probe harboring the binding site in pulldown experiments, followed by mass spec identification of the binding proteins. We identified four members of the NuRD repressor complex among the proteins pulled down. The specific binding of the CHD4 core component of the complex to the human tenascin-W promoter was confirmed by chromatin immunoprecipitation. We therefore speculate that the human tenascin-W gene is silenced by NuRD-mediated chromatin remodeling.
|Committee Members:||Christofori, Gerhard and Bentires-Alj, Mohamed|
|Faculties and Departments:||03 Faculty of Medicine > Departement Biomedizin > Division of Biochemistry and Genetics > Tumor Biology (Christofori)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||101 S.|
|Last Modified:||30 Jun 2016 10:50|
|Deposited On:||16 Oct 2012 13:20|
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