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Ca2+ binding to phosphatidylcholine bilayers as studied by deuterium magnetic resonance : evidence for the formation of a Ca2+ complex with two phospholipid molecules

Altenbach, C. and Seelig, J.. (1984) Ca2+ binding to phosphatidylcholine bilayers as studied by deuterium magnetic resonance : evidence for the formation of a Ca2+ complex with two phospholipid molecules. Biochemistry, Vol. 23, H. 17. S. 3913-3920.

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Official URL: http://edoc.unibas.ch/dok/A5257515

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Abstract

The binding of Ca2+ to bilayer membranes composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was investigated with atomic absorption spectroscopy and deuterium magnetic resonance, leading to the following conclusions. Atomic absorption spectroscopy allowed the determination of the amount of Ca2+ bound to the membrane surface (Cb) at low Ca2+ concentrations (3-100 mM). Simultaneous measurements of the deuterium magnetic resonance spectra of POPC with specifically deuterated choline head groups revealed a linear relationship between the quadrupole splitting and the amount of bound Ca2+. With this calibration, the amount of bound Ca2+ could be determined from the deuterium spectra under conditions where atomic absorption spectroscopy was technically not feasible, i.e., in the concentration range of 0.1-5 M CaCl2. The Ca2+ binding isotherm exhibited saturation behavior. The quadrupole splitting at the saturation limit corresponded to a binding stoichiometry of one Ca2+ per two POPC molecules. The surface charge density (sigma) could be evaluated from the amount of bound Ca2+ and the surface area per POPC molecule. By employing the Gouy-Chapman theory, it was then possible to determine the surface potential (psi 0) and the Ca2+ concentration immediately at the lipid-water interface (CI). With this set of experimental parameters, various models for the mode of Ca2+ binding were tested. A simple partition equilibrium or a Langmuir absorption model could be ruled out. However, a very good fit to the experimental data was obtained by applying the law of mass action in the form Cb/(1 – 2Cb)2 = KCI in which K is the only adjustable parameter.(ABSTRACT TRUNCATED AT 250 WORDS)
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Biophysical Chemistry (Seelig J)
UniBasel Contributors:Seelig, Joachim
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:American Chemical Society
ISSN:0006-2960
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:20
Deposited On:22 Mar 2012 13:18

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