edoc

Gene 68, a new bacteriophage T4 gene which codes for the 17K prohead core protein is involved in head size determination

Keller, B. and Sengstag, C. and Kellenberger, E. and Bickle, T. A.. (1984) Gene 68, a new bacteriophage T4 gene which codes for the 17K prohead core protein is involved in head size determination. Journal of molecular biology, Vol. 180, H. 3. S. 415-430.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A6001806

Downloads: Statistics Overview

Abstract

We have identified the gene for a major component of the prohead core of bacteriophage T4, the 17K protein. The gene, which we call gene 68, lies between genes 67 and 21 in the major cluster of T4 head genes. All of the genes in this region of the T4 genome have overlapping initiation and termination codons with the sequence T-A-A-T-G. We present the DNA sequence of the gene and show that it codes for a protein containing 141 amino acids with an acidic amino-terminal half and a basic carboxyl terminus. Antibodies prepared against the 17K protein were used to show that it is cleaved by the phage-coded gp21 protease during head maturation and that most of the protein leaves the head after cleavage. A frameshift mutation of the gene was constructed in vitro and recombined back into the phage genome. The mutated phages had a drastically reduced burst size and about half of the particles produced were morphologically abnormal, having isometric rather than prolate heads. Thus, the 17K protein is involved in head shape determination but is only semi-essential for T4 growth.
Faculties and Departments:11 Rektorat und Verwaltung > Vizerektorat Forschung
UniBasel Contributors:Sengstag, Christian
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0022-2836
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:11 Oct 2012 15:31
Deposited On:11 Oct 2012 15:16

Repository Staff Only: item control page