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P-element-mediated enhancer detection : an efficient method for isolating and characterizing developmentally regulated genes in Drosophila

Wilson, C. and Pearson, R. K. and Bellen, H. J. and O'Kane, C. J. and Grossniklaus, U. and Gehring, W. J.. (1989) P-element-mediated enhancer detection : an efficient method for isolating and characterizing developmentally regulated genes in Drosophila. Genes & development, Vol. 3, H. 9. S. 1301-1313.

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Official URL: http://edoc.unibas.ch/dok/A5257333

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Abstract

We describe a new approach for identifying and studying genes involved in Drosophila development. Single copies of an enhancer detector transposon, P[1ArB], have been introduced into flies at many different genomic locations. The beta-galactosidase reporter gene in this construct is influenced by a wide range of genomic transcriptional regulatory elements in its vicinity. Our results suggest that a significant proportion of these regulatory sequences are control elements of nearby Drosophila genes. These genes need not be disrupted for their regulatory elements to be identified by P[1ArB]. The P[1ArB] transposon has been designed to facilitate both rapid cloning and deletion analysis of genomic sequences into which it inserts. Therefore, the enhancer detection system is an efficient method of screening for genes primarily on the basis of their expression pattern and then rapidly analyzing those of particular interest at the molecular and genetic levels.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Gehring)
UniBasel Contributors:Gehring, Walter Jakob
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Cold Spring Harbor Laboratory
ISSN:0890-9369
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:19
Deposited On:22 Mar 2012 13:17

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