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Bidirectional correlation of NMR and capillary electrophoresis fingerprints : a new approach to investigating Schistosoma mansoni infection in a mouse model

Garcia-Perez I., and Couto, A. A. and Angulo, S. and Li, J. V. and Utzinger, J. and Ebbels, T. M. and Legido-Quigley C., and Nicholson, J. K. and Holmes, E. and Barbas, C.. (2010) Bidirectional correlation of NMR and capillary electrophoresis fingerprints : a new approach to investigating Schistosoma mansoni infection in a mouse model. Analytical chemistry, Vol. 82, H. 1. S. 203-210.

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Official URL: http://edoc.unibas.ch/dok/A5842829

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Abstract

We demonstrate the statistical integration of nuclear magnetic resonance (NMR) spectroscopy and capillary electrophoresis (CE) data in order to describe a pathological state caused by Schistosoma mansoni infection in a mouse model based on urinary metabolite profiles. Urine samples from mice 53 days post infection with S. mansoni and matched controls were analyzed via NMR spectroscopy and CE. The two sets of metabolic profiles were first processed and analyzed independently and were subsequently integrated using statistical correlation methods in order to facilitate cross assignment of metabolites. Using this approach, metabolites such as 3-ureidopropionate, p-cresol glucuronide, phenylacetylglycine, indoxyl sulfate, isocitrate, and trimethylamine were identified as differentiating between infected and control animals. These correlation analyses facilitated structural elucidation using the identification power of one technique to enhance and validate the other, but also highlighted the enhanced ability to detect functional correlations between metabolites, thereby providing potential for achieving deeper mechanistic insight into the biological process
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Eco System Health Sciences > Health Impact Assessment (Utzinger)
UniBasel Contributors:Utzinger, Jürg
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:American Chemical Society
ISSN:0003-2700
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Sep 2012 07:19
Deposited On:14 Sep 2012 06:51

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