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Evaluation of two long synthetic merozoite surface protein 2 peptides as malaria vaccine candidates

Flueck, C. and Frank, G. and Smith, T. and Jafarshad, A. and Nebie, I. and Sirima, S. B. and Olugbile, S. and Alonso, P. and Tanner, M. and Druilhe, P. and Felger, I. and Corradin, G.. (2009) Evaluation of two long synthetic merozoite surface protein 2 peptides as malaria vaccine candidates. Vaccine : the official journal of the International Society for Vaccines, 27 (20). pp. 2653-2661.

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Official URL: http://edoc.unibas.ch/dok/A5843213

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Abstract

Merozoite surface protein 2 (MSP2) is a promising vaccine candidate against Plasmodium falciparum blood stages. A recombinant 3D7 form of MSP2 was a subunit of Combination B, a blood stage vaccine tested in the field in Papua New Guinea. A selective effect in favour of the allelic family not represented by the vaccine argued for a MSP2 vaccine consisting of both dimorphic variants. An alternative approach to recombinant manufacture of vaccines is the production of long synthetic peptides (LSP). LSP exceeding a length of well over 100 amino acids can now be routinely synthesized. Synthetic production of vaccine antigens cuts the often time-consuming steps of protein expression and purification short. This considerably reduces the time for a candidate to reach the phase of clinical trials. Here we present the evaluation of two long synthetic peptides representing both allelic families of MSP2 as potential vaccine candidates. The constructs were well recognized by human immune sera from different locations and different age groups. Furthermore, peptide-specific antibodies in human immune sera were associated with protection from clinical malaria. The synthetic fragments share major antigenic properties with native MSP2. Immunization of mice with these antigens yielded high titre antibody responses and monoclonal antibodies recognized parasite-derived MSP2. Our results justify taking these candidate poly-peptides into further vaccine development
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Health Systems Research and Dynamic Modelling > Dynamical Modelling (Smith)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Molecular Diagnostics (Felger)
UniBasel Contributors:Felger, Ingrid and Smith, Thomas A. and Tanner, Marcel
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Elsevier ; [Online:] Amsterdam
ISSN:0264-410X
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:03 Nov 2017 11:50
Deposited On:14 Sep 2012 06:41

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