Steiner, Samuel. Cyclic di-GMP-mediated regulation of the Pga exopolysaccharide secretion machinery in "Escherichia coli". 2012, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_10016
In Boehm & Steiner et al. (2009), the molecular principles triggering poly-GlcNAc-dependent biofilm induction in response to subinhibitory concentrations of translation inhibitors were investigated. We present evidence that translational stress at the ribosome causes a SpoT-mediated reduction of ppGpp levels that results in the specific derepression of PgaA, an essential component of the poly-GlcNAc secretion machinery. In addition, we show that c-di-GMP that is mainly produced by the dedicated DGC YdeH strongly stimulates poly-GlcNAc-dependent biofilm formation and upregulates PgaD, another essential component of the secretion machinery. Evidence is provided that ppGpp and c-di-GMP play synergistic roles in controlling the poly-GlcNAc secretion machinery on a post-transcriptional level.
In the second and main part of this work, we show that the Pga machinery, which is involved in the production and secretion of poly-GlcNAc, is allosterically activated by c-di-GMP and we identify the inner membrane-bound PgaCD complex composed of the glycosyltransferase PgaC and the small protein PgaD as a novel type c-di-GMP receptor.
|Committee Members:||Bumann, Dirk|
|Faculties and Departments:||05 Faculty of Science > Departement Biozentrum > Growth & Development > Molecular Microbiology (Jenal)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||162 S.|
|Last Modified:||30 Jun 2016 10:49|
|Deposited On:||24 Aug 2012 11:43|
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