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B/T cell ratio of rabbit peripheral blood lymphocytes : influence of separation technique on results

Walstra, K. and Gratwohl, A. and Riederer, I. and Speck, B.. (1985) B/T cell ratio of rabbit peripheral blood lymphocytes : influence of separation technique on results. Journal of immunological methods, Vol. 79, H. 1. S. 143-147.

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Official URL: http://edoc.unibas.ch/dok/A5844547

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Abstract

Spontaneous in vitro T cell rosette formation at room temperature leading to enrichment of B cells has been reported. We tested 10 individual New Zealand White rabbits sequentially, separating the lymphocytes either at room temperature or at 37 degrees C. T cells are lost constantly at room temperature but to a lesser extent at 37 degrees C. The determination of the yield of lymphocytes after Ficoll separation gives the best control for the accuracy of the results. If lymphocytes are separated at 37 degrees C and if the yield of lymphocytes is greater than 45%, the variation in T cells is small and their number is constant between 62 and 74%. These data show that the reported wide range of T cells in rabbit peripheral blood is due to methodological errors and not inherent in the rabbit.
Faculties and Departments:03 Faculty of Medicine > Departement Public Health > Pharmazeutische Medizin ECPM > Pharmazeutische Medizin (Szucs)
UniBasel Contributors:Matter-Walstra, Klazien Wietske
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0022-1759
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:08 Jun 2012 06:53
Deposited On:08 Jun 2012 06:30

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