Scheckel, Claudia. mRNA regulation in the "C. elegans" germ line. 2011, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_9687
We found that translation initiation of many germline mRNAs is repressed, and that GLD-1 globally represses translation initiation of its targets. Importantly, we revealed an additional role of GLD-1 in stabilizing a large number of its bound mRNAs, suggesting that GLD-1 plays a central role in maternal mRNA storage. While we could not detect an interaction between GLD-1 and translation initiation factors, we observed that GLD-1 associates with components of a conserved germline RNP complex. These components include the polyA binding protein (PABP), Y-box proteins, the Sm-like protein CAR-1 and the DDX6 helicase CGH-1, which has recently been implicated in maternal mRNA protection. Interestingly we found that while CGH-1 does not influence the translational repression of investigated GLD-1 targets, CGH-1 and GLD-1 stabilize a common set of transcripts. Remarkably, these co-regulated messages nearly exclusively encode for mRNAs that are required for the oocyte-to-embryo transition. We therefore propose a two-step model where GLD-1 binding prevents translation initiation and primes many targets for CGH-1-dependent mRNA stabilization, ultimately leading to mRNA storage.
|Committee Members:||Ephrussi, Anne and Ciosk, Rafal|
|Faculties and Departments:||09 Associated Institutions > Friedrich Miescher Institut FMI|
|Bibsysno:||Link to catalogue|
|Number of Pages:||135 S.|
|Last Modified:||30 Jun 2016 10:42|
|Deposited On:||21 Dec 2011 15:32|
Repository Staff Only: item control page