Witmer, Kathrin. Transcriptional regulation of virulence gene families in "Plasmodium falciparum". 2011, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_9698
With this work, I provide deeper insights into the transcriptional regulation of virulence gene families in P. falciparum by using transfection-based approaches. We functionally identified autonomous cis-acting var promoter elements including an upstream activating sequence that is essential for promoter activation. Notably, an element downstream of the transcriptional start site determines mutually exclusive locus recognition. Further, I used comparative transcriptional profiling to show that mutually exclusive expression is restricted to the var gene family and is not used in the transcription of other subtelomeric gene families. I show for the first time that knock-down of endogenous var gene transcription is also conferred by promoters of a var gene subfamily that is implicated in severe malaria. Taken together, this work provides important insight into the mechanisms involved in the regulation of virulence gene families and antigenic variation in P. falciparum. Moreover, the findings presented here are consistent with a novel mechanism of mutually exclusive gene choice in eukaryotes.
|Committee Members:||Gilberger, Tim-Wolf|
|Faculties and Departments:||09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Gene Regulation (Voss)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||168 S.|
|Last Modified:||30 Jun 2016 10:42|
|Deposited On:||16 Dec 2011 08:10|
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