Rossel, Thibaud. Artificial phosphate transferases and hydrogen tranferases based on Biotin-Streptavidin technology. 2011, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_9642
hydrogenases. The second part of this thesis presents the incorporation of catalytically active biotinylated complexes within streptavidin, creating artificial metalloenzymes for the transfer hydrogenation of prochiral ketones. The optimization of the second coordination sphere, based on a crystal structure of the active hybrid catalyst and successive rounds of saturation mutagenesis at selected positions termed “designed evolution”, afforded highly active and selective catalysts for the reduction of challenging ketone substrates. The well-defined active site of the hybrid catalysts can be redesigned for the reduction of novel substrates such as imines to create the first example of an artificial imine reductase.
|Advisors:||Ward, Thomas R.|
|Committee Members:||Wennemers, Helma|
|Faculties and Departments:||05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||181 S.|
|Last Modified:||30 Jun 2016 10:42|
|Deposited On:||02 Nov 2011 07:56|
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