Topf, Ulrike. Investigations on the functions ot "ten-1" during embryonic development in "Caenorhabditis" elegans. 2011, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_9585
The advantage of C. elegans as a model organism to investigate the function and mechanism of action of teneurins is that the C. elegans genome codes only for a single ortholog of the vertebrate teneurins. Two different promoters give rise to at least two different transcript versions. The promoters show distinct patterns of activity in the mesoderm and in the ectoderm of the worm. Two independent deletion mutants are characterized as null mutations. Knock-down by RNAi and analysis of the null mutants revealed that ten-1 is essential for embryonic development but also for postembryonic events like larval development and reproduction. Interestingly, investigations on potential genetic interaction partners of ten-1 uncovered functional redundancy between ten-1 and basement membrane associated genes coding for homologs of integrin, dystroglycan, laminin, nidogen and perlecan. Co-staining of chicken teneurin and laminin in some tissues during embryonic development might point to an evolutionary conserved function of teneurins associated with the basement membrane.
In this study I used a genome wide approach to obtain a comprehensive list of potential genetic interaction partners of ten-1. I characterized the interaction with phy-1 in more detail. phy-1 is conserved among the phyla and codes for the catalytical domain of the collagen-modifying enzyme prolyl 4-hydroxylase. I found that loss of phy-1 in the ten-1 null mutant background enhances embryonic lethality significantly. Worms die during late elongation because of loss of connectivity between muscles, epidermis and the separating basement membrane. In addition, I present evidence that the function of PHY-1 modifying basement membrane collagen IV is conserved also in C. elegans.
|Committee Members:||Affolter, Markus|
|Faculties and Departments:||05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell Biology (Affolter)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||128 S.|
|Last Modified:||30 Jun 2016 10:42|
|Deposited On:||02 Sep 2011 09:48|
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