Kilchert, Cornelia. mRNA localization and turnover in mutants of the small GTPase Arf1p of "Saccharomyces cerevisiae". 2010, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_9195
As one of the transcripts was found to localize asymmetrically in wild-type cells, its transport was characterized in greater detail. A model is described how this mRNA is targeted to its unusual localization, the distal pole of the mother cell, by cotranslational coupling to actin cables that undergo retrograde flow.
Finally, a series of experiments is described that help to define what distinguishes multiple P bodies induced in secretory mutants from the few bright granules that are observed after glucose starvation. It is shown that the appearance of multiple P bodies is linked to Ca2+, and a set of proteins is identified that is strictly required for formation of Ca2+-induced P bodies but is dispensable for P body induction under other conditions. In addition, it is demonstrated that different pathways for P body induction directly regulate P body number. In a final set of experiments, it is shown that P bodies are stably linked to the ER under all conditions tested.
|Committee Members:||Spiess, Martin|
|Faculties and Departments:||05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spang)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||184 S.|
|Last Modified:||30 Jun 2016 10:41|
|Deposited On:||29 Dec 2010 09:02|
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