Na, Kyung-Jae. Function of Gata-2 in thymic epithelial cells : a transcription factor identified from gene expression analysis of endodermal cells committed to thymic epithelial cell fate. 2010, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_9254
Gata-2, a transcription factor, known to be involved in hematopoiesis, was the only gene of which its expression was detected by gene chip data, RT-PCR and whole mount in situ hybridization. These results identified Gata-2 as a novel candidate that might be involved in the thymic epithelial cell development. To characterize the function of Gata-2 in thymus development, Gata-2 was specifically deleted in thymic epithelial cells using Foxn1-Cre. The thymi of 3, 6, 13, and 25 weeks old mice were removed and detailed studies were performed. FACS analysis of these thymi revealed an increased thymus cellularity in DN1-DN4, CD4, and CD8 in 6 weeks old thymi and onwards. The thymus architecture which was analyzed by H&E and immunohistochemistry (UEA-1, CK8, CK18, ERTR7) was unaffected when Gata-2 was deleted in TECs. The assessment of TEC population of Gata-2 KO mice did not show any difference. But the gene expression analysis of Gata-2 deficient TECs for the genes c-Jun, CXCL-12, CCL-25, IL-7, c-Fos, c-kit ligand, Edn-1, Edn-Ra, und Edn-Rb showed that CXCL-12 and c-kit ligand were higher expressed. CXCL-12 is involved in homing of T-cell precursors while c-kit L is involved in survival and proliferation of T-cell precursors. 7
In conclusion, Gata-2 might negatively regulate the transcription of CXCL-12 and c-kit ligand. A lack of Gata-2 expression in thymic epithelial cells, therefore, might lead to an increased T-cell precursor attraction and survival/proliferation, thus, explaining the higher cellularity observed in thymus of Gata-2 deficient mice.
|Advisors:||Holländer, Georg A.|
|Committee Members:||Palmer, Ed|
|Faculties and Departments:||03 Faculty of Medicine > Bereich Kinder- und Jugendheilkunde (Klinik) > Kinder- und Jugendheilkunde (UKBB) > Pädiatrische Immunologie (Holländer)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||110 S.|
|Last Modified:||30 Jun 2016 10:41|
|Deposited On:||27 Dec 2010 10:12|
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