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Rotational diffusion of band 3 in the red cell membrane : measurements using triplet probes

Cherry, R. J. and Nigg, E.. (1979) Rotational diffusion of band 3 in the red cell membrane : measurements using triplet probes. Progress in clinical and biological research, vol. 30. S. 475-481.

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Official URL: http://edoc.unibas.ch/dok/A5249536

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Abstract

Rotational diffusion of band 3 in the human erythrocyte membrane has been investigated using the technique of flash photolysis. Membranes are labeled with the triplet probe eosin isothiocyanate; most of the label is found to be associated with band 3. Rotation is measured by observing the decay of dichroism of flash-induced absorbance changes arising from the eosin probe. The results indicate that band 3 rotates about an axis normal to the plane of the membrane with a diffusion coefficient in the order of 1,000 sec-1. Removal of spectrin has no observable effect on the rotation of band 3 at pH 7.6. Essentially similar results are obtained with a different probe, iodoacetamidoeosin. Both eosin derivatives are strong inhibitors of anion transport. Illumination of eosin-labeled ghosts causes a rapid loss of acetylcholinesterase activity but this can be prevented by prior displacement of oxygen in the sample by argon.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum
05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell Biology (Nigg)
UniBasel Contributors:Nigg, Erich A.
Item Type:Article, refereed
Bibsysno:Link to catalogue
Note:Also published in: Normal and abnormal red cell membranes. - New York : A.R. Liss, 1979. - S. 475-481 -- Publication type according to Uni Basel Research Database: Journal article
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Last Modified:07 Aug 2015 12:05
Deposited On:22 Mar 2012 13:59

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